CD43 antibody LS-C13384 is an unconjugated mouse monoclonal antibody to human CD43 (SPN). Validated for Flow and IHC.
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin
- IHC - Frozen
- Flow Cytometry
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SPN / CD43 antibody was raised against stimulated human leukocytes.
Recognizes CD43 (also known as leukosialin, sialophorin or leukocyte sialoglycoprotein) at 95kD, 115kD or 135kD, depending on the degree of glycosylation. Species cross-reactivity: Human. Does not react with Rat.
Suitable for use in Flow Cytometry, Immunohistochemistry. Immunohistochemistry (Frozen & formalin/paraffin): 1-2 ug/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Tonsil or a lymph node.
PBS, pH 7.4, 15 mM Sodium Azide, 0.2% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.