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Antibodies > SPN / CD43 > LS-C343958
 
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Catalog Number Size Price
LS-C343958-10 10 µg $318 
LS-C343958-100 100 µg (0.5 mg/ml) $470 
SPN / CD43 Antibody - IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SPN / CD43 Antibody - IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SPN / CD43 Antibody - IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in immunocytochemical section of K562 Cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SPN / CD43 Antibody - CD43 antibody IHC-paraffin: Mouse Spleen Tissue.
SPN / CD43 Antibody - CD43 antibody IHC-paraffin: Rat Spleen Tissue.
SPN / CD43 Antibody - CD43 antibody IHC-paraffin: Human Tonsil Tissue.
SPN / CD43 Antibody - IF analysis of CD43 using anti-CD43 antibody CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD43 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SPN / CD43 Antibody - Western blot analysis of CD43 using anti-CD43 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD43 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD43 at approximately 115KD. The expected band size for CD43 is at 40KD.
SPN / CD43 Antibody - CD43 antibody Western blot. All lanes: Anti CD43 at 0.5 ug/ml. WB: Recombinant Human CD43 Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.
SPN / CD43 Antibody - Flow Cytometry analysis of P3NSI cells using anti-CD43 antibody. Overlay histogram showing P3NSI cells stained with anti-CD43 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
SPN / CD43 Antibody - Flow Cytometry analysis of BRL cells using anti-CD43 antibody. Overlay histogram showing BRL cells stained with anti-CD43 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
SPN / CD43 Antibody - Flow Cytometry analysis of HL-60 cells using anti-CD43 antibody. Overlay histogram showing HL-60 cells stained with anti-CD43 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
SPN / CD43 Antibody - IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SPN / CD43 Antibody - IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SPN / CD43 Antibody - IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in immunocytochemical section of K562 Cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SPN / CD43 Antibody - CD43 antibody IHC-paraffin: Mouse Spleen Tissue.
SPN / CD43 Antibody - CD43 antibody IHC-paraffin: Rat Spleen Tissue.
SPN / CD43 Antibody - CD43 antibody IHC-paraffin: Human Tonsil Tissue.
SPN / CD43 Antibody - IF analysis of CD43 using anti-CD43 antibody CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD43 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SPN / CD43 Antibody - Western blot analysis of CD43 using anti-CD43 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD43 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD43 at approximately 115KD. The expected band size for CD43 is at 40KD.
SPN / CD43 Antibody - CD43 antibody Western blot. All lanes: Anti CD43 at 0.5 ug/ml. WB: Recombinant Human CD43 Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.
SPN / CD43 Antibody - Flow Cytometry analysis of P3NSI cells using anti-CD43 antibody. Overlay histogram showing P3NSI cells stained with anti-CD43 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
SPN / CD43 Antibody - Flow Cytometry analysis of BRL cells using anti-CD43 antibody. Overlay histogram showing BRL cells stained with anti-CD43 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
SPN / CD43 Antibody - Flow Cytometry analysis of HL-60 cells using anti-CD43 antibody. Overlay histogram showing HL-60 cells stained with anti-CD43 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human SPN / CD43 Antibody (aa272‑400, IHC, WB) LS‑C343958

Polyclonal Rabbit anti‑Human SPN / CD43 Antibody (aa272‑400, IHC, WB) LS‑C343958

Submit Review LSBio Guarantee
Antibody:
SPN / CD43 Rabbit anti-Human Polyclonal (aa272-400) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343958-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SPN / CD43 Rabbit anti-Human Polyclonal (aa272-400) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD43 antibody LS-C343958 is an unconjugated rabbit polyclonal antibody to CD43 (SPN) (aa272-400) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human SPN / CD43
Synonyms
SPN | GPL115 | Leukosialin | Leukocyte sialoglycoprotein | Galactoglycoprotein | GALGP | Sialophorin | LSN | CD43 | CD43 antigen
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.
Epitope
aa272-400
Specificity
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPN / CD43
P16150 NM_003123 NP_003114.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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