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Catalog Number Size Price
LS-B9322-200 200 µl $460 
SPI1 / PU.1 Antibody - Anti-SPI1 / PU.1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
SPI1 / PU.1 Antibody - Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody. NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SPI1 immunoreactivity is localized to Nucleus significantly.
SPI1 / PU.1 Antibody - SPI1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The SPI1 antibody detected the SPI1 protein (arrow).
SPI1 / PU.1 Antibody - Anti-SPI1 / PU.1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
SPI1 / PU.1 Antibody - Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody. NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SPI1 immunoreactivity is localized to Nucleus significantly.
SPI1 / PU.1 Antibody - SPI1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The SPI1 antibody detected the SPI1 protein (arrow).
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IHC‑plus™ Polyclonal Rabbit anti‑Human SPI1 / PU.1 Antibody (aa217‑246, IHC, IF, WB) LS‑B9322

IHC‑plus™ Polyclonal Rabbit anti‑Human SPI1 / PU.1 Antibody (aa217‑246, IHC, IF, WB) LS‑B9322

Note: This antibody replaces LS-C161449
Antibody:
SPI1 / PU.1 Rabbit anti-Human Polyclonal (aa217-246) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B9322-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SPI1 / PU.1 Rabbit anti-Human Polyclonal (aa217-246) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PU.1 antibody LS-B9322 is an unconjugated rabbit polyclonal antibody to human PU.1 (SPI1) (aa217-246). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SPI1 / PU.1
Synonyms
SPI1 | 31 kDa-transforming protein | 31 kDa transforming protein | PU.1 | SFPI1 | SPI-1 | SPI-1 proto-oncogene | SPI-A | Transcription factor PU.1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa217-246
Specificity
This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C for up to 6 months. For long-term storage, store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPI1 / PU.1
P17947 NM_003120 NP_003111.2

Validation

SPI1 / PU.1 Antibody - Anti-SPI1 / PU.1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPI1 / PU.1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SPI1 / PU.1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ SPI1 / PU.1 Antibody (aa217-246) for IHC, IF/Immunofluorescence, WB/Western LS-B9322 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024