LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-SPI1 / PU.1 Antibody (aa20-70) LS-C148795

Ordering

Wt. Vol. Conc. Price
100 µg - - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SPI1 / PU.1 Antibodies

Anti-SPI1 / PU.1 Antibody (FITC) LS-C317499
Rabbit Polyclonal (IgG) to Human SPI1 / PU.1
Human
IHC - Paraffin, Western blot, ELISA
FITC Conjugated
Immunohistochemistry - Paraffin Image
Anti-SPI1 / PU.1 Antibody (N-Terminus) LS-C38665
Rabbit Polyclonal (IgG) to Human SPI1 / PU.1
Human, Monkey, Mouse, Pig
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Unconjugated
Anti-SPI1 / PU.1 Antibody IHC-plus™ LS-B4206
Rabbit Polyclonal to Human SPI1 / PU.1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SPI1 / PU.1
Human, Monkey, Mouse, Bovine, Dog, Horse
Western blot, Flow Cytometry
Unconjugated

Details

Human SPI1 / PU.1
Rabbit
Human, Monkey, Mouse, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (4 - 6 µg/ml)
  • Flow Cytometry

Specificity and Use

SPI1 / PU.1 antibody was raised against amino acids 20-70 of human PU.1 were used as the immunogen.
aa20-70
Human SPI1 / PU.1.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide
Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
For research use only.

About SPI1 / PU.1

P17947 NM_003120 NP_003111.2

SPI1 Antibody, 31 kDa-transforming protein Antibody, 31 kDa transforming protein Antibody, PU.1 Antibody, SFPI1 Antibody, SPI-1 Antibody, SPI-1 proto-oncogene Antibody, SPI-A Antibody, Transcription factor PU.1 Antibody

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.

Western blot

Western blot
Western blot of PU.1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148795 at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test.

Flow Cytometry

Flow Cytometry
Intracellular flow cytometry of PU.1 in mouse RAW cells (top) and PMA treated (20 ng/ml, overnight) human ThP1 cells (bottom) using 1.25 ug/10^6 cells. Green peak represents isotype control, ; red represents anti-PU.1 antibody.

Requested From: United States
Date Requested: 12/11/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number