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Catalog Number Size Price
LS-C112683-100 100 µg (0.5 mg/ml) $503 
Antibody

Polyclonal Goat anti‑Human SPAG16 Antibody (aa139‑152, IHC, IF) LS‑C112683

Polyclonal Goat anti‑Human SPAG16 Antibody (aa139‑152, IHC, IF) LS‑C112683

Antibody:
SPAG16 Goat anti-Human Polyclonal (aa139-152) Antibody
Application:
IHC, IF, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C112683-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SPAG16 Goat anti-Human Polyclonal (aa139-152) Antibody
Application:
IHC, IF, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SPAG16 antibody LS-C112683 is an unconjugated goat polyclonal antibody to human SPAG16 (aa139-152). Validated for IF, IHC and Peptide-ELISA.
Target
Human SPAG16
Synonyms
SPAG16 | Pf20 protein homolog | WDR29 | PF20 | Sperm associated antigen 16 | WD repeat domain 29
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KGVTELRTVGNVPD, from the internal region of the protein sequence according to NP_078808.3NP_001020607.1.
Epitope
aa139-152
Specificity
Human SPAG16. This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
Applications
  • IHC
  • Immunofluorescence (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.8kDa according to NP_078808.3 and of 20.6kDa according to NP_001020607.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPAG16
Q8N0X2 NM_024532 NP_078808.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024