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Anti-SOX4 Antibody (aa86-114) LS-C97708

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SOX4 Antibodies

Anti-SOX4 Antibody LS-C75932
Mouse Monoclonal (IgG2a) to Human SOX4
Human
Western blot
Unconjugated
Anti-SOX4 Antibody (aa86-114) LS-C97708
Rabbit Polyclonal to Human SOX4
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SOX4
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human SOX4
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

SOX4 antibody was raised against kLH-conjugated synthetic peptide between amino acids 86-114 from N-terminal region of human SOX4.
LS-E6828 - Lyophilized - 100 µg - $145.00
aa86-114

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SOX4

Q06945 NM_003107 NP_003098.1

SOX4 Antibody, EVI16 Antibody, SRY-related HMG-box gene 4 Antibody, Transcription factor SOX-4 Antibody

This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin).

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with SOX4 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly.

Western blot

Western blot
Western blot of SOX4 antibody in A2058 cell line lysates (35 ug/lane). SOX4 (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
SOX4 Antibody flow cytometry of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/10/2016

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