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Antibodies > SOX2 > LS-C96467
 
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Catalog Number Size Price
LS-C96467-400 400 µl $393 
SOX2 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
SOX2 Antibody - Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.
SOX2 Antibody - Fluorescent image of A549 cell stained with SOX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SOX2 immunoreactivity is localized to Nucleus significantly.
SOX2 Antibody - Western blot of SOX2 (arrow) using mouse monoclonal SOX2 antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)
SOX2 Antibody - Western blot of SOX2 Antibody by SOX2 recombinant protein. SOX2(arrow) was detected using the purified antibody.
SOX2 Antibody - Western blot of lysate from SOX2 protein, using SOX2 Antibody. Antibody was diluted at 1:4000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.
SOX2 Antibody - Flow cytometric of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
SOX2 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
SOX2 Antibody - Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.
SOX2 Antibody - Fluorescent image of A549 cell stained with SOX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SOX2 immunoreactivity is localized to Nucleus significantly.
SOX2 Antibody - Western blot of SOX2 (arrow) using mouse monoclonal SOX2 antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)
SOX2 Antibody - Western blot of SOX2 Antibody by SOX2 recombinant protein. SOX2(arrow) was detected using the purified antibody.
SOX2 Antibody - Western blot of lysate from SOX2 protein, using SOX2 Antibody. Antibody was diluted at 1:4000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.
SOX2 Antibody - Flow cytometric of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
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Monoclonal Mouse anti‑Human SOX2 Antibody (clone 57CT23.3.4, IHC, IF, WB) LS‑C96467

Monoclonal Mouse anti‑Human SOX2 Antibody (clone 57CT23.3.4, IHC, IF, WB) LS‑C96467

Submit Review LSBio Guarantee
Antibody:
SOX2 Mouse anti-Human Monoclonal (57CT23.3.4) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C96467-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SOX2 Mouse anti-Human Monoclonal (57CT23.3.4) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SOX2 antibody LS-C96467 is an unconjugated mouse monoclonal antibody to human SOX2. Validated for Flow, IF, IHC and WB.
Target
Human SOX2
Synonyms
SOX2 | MCOPS3 | Transcription factor SOX2 | ANOP3 | Transcription factor SOX-2 | SRY-related HMG-box gene 2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
57CT23.3.4
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:200 - 1:2000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Presentation
Liquid
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SOX2
P48431 L07335 AAA35997.1

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SOX2 ELISA Kit
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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