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Anti-SOX2 Antibody (clone 57CT23.3.4) LS-C96467

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SOX2 Antibodies

Anti-SOX2 Antibody (aa29-78) LS-C31802
Rabbit Polyclonal (IgG) to Human SOX2
Human, Monkey, Mouse, Rat, Bat, Bovine, Goat, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-SOX2 Antibody IHC-plus™ LS-B3829
Goat Polyclonal to Human SOX2
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Sheep
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-SOX2 Antibody (aa113-127) LS-C141816
Rabbit Polyclonal (IgG) to Human SOX2
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 57CT23.3.4] (IgG1) to Human SOX2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human SOX2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [57CT23.3.4]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:200 - 1:2000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

SOX2 antibody was raised against sOX2 recombinant protein.

Packaging

Liquid
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SOX2

P48431 L07335 AAA35997.1

SOX2 Antibody, MCOPS3 Antibody, Transcription factor SOX2 Antibody, ANOP3 Antibody, Transcription factor SOX-2 Antibody, SRY-related HMG-box gene 2 Antibody

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity).

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.

Immunofluorescence

Immunofluorescence
Fluorescent image of A549 cell stained with SOX2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SOX2 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
Western blot of lysate from SOX2 protein, using SOX2 Antibody. Antibody was diluted at 1:4000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot
Western blot of SOX2 Antibody by SOX2 recombinant protein. SOX2(arrow) was detected using the purified antibody.

Western blot

Western blot
Western blot of SOX2 (arrow) using mouse monoclonal SOX2 antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)

Flow Cytometry

Flow Cytometry
Flow cytometric of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
 

Requested From: United States
Date Requested: 12/2/2016

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