Target
Human SORBS1 / Ponsin
Synonyms
SORBS1 | C-Cbl associated protein | C-Cbl-associated protein | CAP | FLAF2 | KIAA0894 | KIAA1296 | R85FL | SH3 domain protein 5 | SH3-domain protein 5 (ponsin) | SH3P12 | SORB1 | Fas-ligand associated factor 2 | Ponsin | SH3D5
Reactivity
Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-GTFPGNYVKPLYL, from the C-Terminus of protein sequence according to NP_006425.2NP_056200.1NP_001030126.1NP_001030127.1NP_001030128.1NP_079267.1NP_001030129.1.
Specificity
Human SORBS1. This antibody is expected to recognize all seven reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1).
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
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Usage
Immunohistochemistry: LS-B1762 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1762 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee