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Anti-SNAP25 Antibody (aa193-206) IHC-plus™ LS-B2392

Note: This antibody replaces LS-C20213

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SNAP25 Antibodies

Anti-SNAP25 Antibody (aa183-197, clone B371M) LS-C124563
Mouse Monoclonal [clone B371M] (IgG) to Human SNAP25
Human
IHC, ELISA
Unconjugated
Anti-SNAP25 Antibody (aa183-197, clone 9L651) LS-C124564
Mouse Monoclonal [clone 9L651] (IgG) to Human SNAP25
Human, Rat
IHC, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human SNAP25
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SNAP25
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • ELISA (1:32000)

Specificity and Use

SNAP25 antibody was raised against synthetic peptide C-DEANQRATKMLGSG from the C-terminus of human SNAP25 (NP_003072.2; NP_570824.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%); Frog (93%); Xenopus (86%).
LS-E25868 - Lyophilized - 100 µg - $145.00
aa193-206
Human SNAP25. This antibody is expected to recognise both reported isoforms NP_003072 and NP_570824.
Immunohistochemistry: LS-B2392 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2392 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SNAP25

P60880 NM_003081 NP_003072.2

SNAP25 Antibody, BA416N4.2 Antibody, DJ1068F16.2 Antibody, SNAP Antibody, SUP Antibody, Super protein Antibody, RIC4 Antibody, SEC9 Antibody, SNAP-25 Antibody, RIC-4 Antibody

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE.

Immunohistochemistry

Immunohistochemistry
Anti-SNAP25 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2392 concentration 5 ug/ml.

Western blot

Western blot
(0.03 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/6/2016

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