Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
SMURF1 antibody was raised against synthetic peptide derived from an internal region of human Smurf1 (Smad ubiquitination regulatory factor 1, Smad-specific E3 ubiquitin ligase 1, Smad-ubiquitin E3 ligase Smurf1-beta).
Reacts with human and mouse Smurf1. On Western blots, it identifies the target band at ~100kD. Reactivity has been confirmed with human A375, NGP96, HepG2, MeWo cell lysates, and mouse liver and testis homogenates. Based on amino acid sequence homology, reactivity with rat may also be observed.
Suitable for use in Western Blot. Western Blot: 1-3 ug/ml.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA.