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Anti-SMO / Smoothened Antibody (N-Terminal) IHC-plus™ LS-A2666

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
Inquire for larger quantities
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Most Popular SMO / Smoothened Antibodies

Anti-SMO / Smoothened Antibody (N-Terminal) IHC-plus™ LS-A2666
Rabbit Polyclonal to Human SMO / Smoothened
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed In Stock In Stock Publications Publications (4)
Rabbit Polyclonal to Human SMO / Smoothened
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated

Details

Human SMO / Smoothened
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot

Specificity and Use

SMO / Smoothened antibody was raised against synthetic 15 amino acid peptide from N-terminal extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).
LS-E28591 - Liquid - 50 µg - $145.00
N-Terminal
Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A2666 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2666 was determined to be 10 ug/ml.

Packaging

PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
For research use only.

Publications (4)

1
Vertebrate Smoothened functions at the primary cilium. Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DY and Reiter JF. Nature. 2005 437:1018-21. (ICC; Mouse, Dog) [PubMed:16136078]
2
Sonic and desert hedgehog signaling in human fetal prostate development. Zhu G, Zhau HE, He H, Zhang L, Shehata B, Wang X, Cerwinka WH, Elmore J, He D. The Prostate. 2007 May;67:674-84. [Full Text Article] [PubMed:17342747]
3
Primary cilia modulate Ihh signal transduction in response to hydrostatic loading of growth plate chondrocytes. Shao YY, Wang L, Welter JF, Ballock RT. Bone. 2012 Jan;50:79-84. [Full Text Article] [PubMed:21930256] [PMC:PMC3246537]
4
Divergence of hedgehog signal transduction mechanism between Drosophila and mammals. Varjosalo M, Li SP, Taipale J. Developmental cell. 2009 Feb;10:177-86. [Full Text Article] [PubMed:16459297]
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About SMO / Smoothened

Q99835 NM_005631 NP_005622.1

SMO Antibody, Frizzled family member 11 Antibody, FZD11 Antibody, Gx Antibody, Protein Gx Antibody, SMOH Antibody, Smoothened Antibody, Smoothened homolog Antibody

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas.

Immunohistochemistry

Immunohistochemistry
Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
 

Requested From: United States
Date Requested: 12/10/2016

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