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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SMEK1 Antibody (aa645‑657) LS‑C55486 Best Seller
SMEK1 antibody LS-C55486 is an unconjugated goat polyclonal antibody to SMEK1 from human, mouse, bat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C55486-100
100 µg
Unavailable

Popular SMEK1 Products

Detection of Mouse PPP4R3 Alpha by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-PPP4R3 Alpha used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human PPP4R3 Alpha by Immunohistochemistry and Immunofluorescence. Sample: FFPE sections of human colon carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti-PPP4R3 Alpha used at a dilution of 1:200 (1 ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry of paraffin-embedded human lung cancer tissue.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Immunohistochemistry - Paraffin Image
Species: Human
Applications: IHC, Western blot

Product Description

SMEK1 antibody LS-C55486 is an unconjugated goat polyclonal antibody to SMEK1 from human, mouse, bat and other species. Validated for Peptide-ELISA and WB.
About SMEK1
Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. NM_032560 AAG39284.1

SMEK1 Antibody, FLFL1 Antibody, KIAA2010 Antibody, MSTP033 Antibody, SMEK homolog 1 Antibody, Smk1 Antibody, PP4R3A Antibody, PPP4R3A Antibody, Smk-1 Antibody


Specifications

Target
Human SMEK1
Host
Goat
Reactivity
Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:16000) & (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Immunogen
SMEK1 antibody was raised against synthetic peptide C-EQQRERQDNPKLD from an internal region of human SMEK1 (NP_115949.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Stickleback, Pufferfish, Zebrafish (100%); Xenopus (92%).
Blocking Peptide
LS-E26438 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55486. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa645-657
Specificity
Human SMEK1.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293 overexpressing SMEK1 (RC221040) and probed with (mock transfection in first lane).
HEK293 overexpressing SMEK1 (RC221040) and probed with (mock transfection in first lane).

Western blot

Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SMEK1 (RC221040) and probed with (mock transfection in first lane).
HEK293 overexpressing SMEK1 (RC221040) and probed with (mock transfection in first lane).

Western blot

Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SMEK1 (RC221040) and probed with (mock transfection in first lane).
HEK293 overexpressing SMEK1 (RC221040) and probed with (mock transfection in first lane).

Western blot

Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SMEK1 (RC221040) and probed with (mock transfection in first lane).
HEK293 overexpressing SMEK1 (RC221040) and probed with (mock transfection in first lane).

Western blot

Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/22/2018

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