Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
SMC1A / SMC1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 916-927 of human SMC1 (C-ETKLEQKRSDRH) with a N-terminal cysteine. The immunizing sequence is identical in rat, bovine and Xenopus and has 11/12 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Pig, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (100%); Trichina worm (83%).
Recognizes SMC1, Mr 150kD. Additional proteins are detected at Mr 45kD and 140kD. All three proteins are competed by the immunizing peptide. Species cross-reactivity: Human. Predicted to cross-react with rat, bovine and Xenopus based on sequence homology.
Western Blot: 2 ug/ul.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain.