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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SMARCA3 / HLTF Antibody (N‑Terminus) LS‑C80187
HLTF antibody LS-C80187 is an unconjugated rabbit polyclonal antibody to HLTF (SMARCA3) from human, mouse, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C80187-50
50 µg
Unavailable

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Product Description

HLTF antibody LS-C80187 is an unconjugated rabbit polyclonal antibody to HLTF (SMARCA3) from human, mouse, bat and other species. Validated for WB.
About SMARCA3 / HLTF
Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Q14527 NM_003071 NP_003062.2

HLTF Antibody, HIP116 Antibody, HIP116A Antibody, HLTF1 Antibody, SMARCA3 Antibody, Sucrose nonfermenting-like 3 Antibody, ZBU1 Antibody, RING finger protein 80 Antibody, SNF2L3 Antibody, RNF80 Antibody, SNF2-like 3 Antibody


Specifications

Target
Human SMARCA3 / HLTF
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
SMARCA3 / HLTF antibody was raised against synthetic peptide from N-Terminus of human HLTF (Q14527, NP_003062). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig (100%); Galago (92%); Mouse (76%).
Blocking Peptide
LS-E13725 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80187. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human HLTF
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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