Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
SMAD7 Antibody, CRCS3 Antibody, HSMAD7 Antibody, MAD homolog 7 Antibody, SMAD 7 Antibody, MADH7 Antibody, MADH8 Antibody, Mothers against DPP homolog 7 Antibody, Mothers against DPP homolog 8 Antibody, MAD homolog 8 Antibody, SMAD family member 7 Antibody
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation.
Smad7 in Human Liver Cancer Tissue. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 ug/ml Human/Mouse/Rat Smad7 Monoclonal Antibody overnight at 4°C. Tissue was stained with Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).
Smad7 in Human Liver. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody at 25 ug/ml overnight at 4°C. Tissue was stained using Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.
Detection of Human/Mouse/Rat Smad7 by Western Blot. Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 ug/ml of Human/Mouse/Rat Smad7 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Smad7 at approximately 50 kD (as indicated). This experiment was conducted under reducing conditions.
Requested From: United States
Date Requested: 12/4/2016