Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
SMAD2 antibody was raised against fusion protein corresponding to amino acids 186-273 of human Smad2.
Recognizes human Smad2 Mr 55-60kD and Smad3 Mr 50kD as determined by using Smad2 and Smad3 transfected Cos cells. Species cross-reactivity: Predicted to cross-react with mouse and rat based on conservation of immunogen sequence.
Suitable for use in Western Blot. Western Blot: 1:1000-1:2000 detects Smad2/3 in RIPA lysates of HepG2 cells. An additional unknown band was detected at Mr 97kD in the lysate.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
SMAD2 Antibody, HMAD-2 Antibody, HSMAD2 Antibody, JV18-1 Antibody, Mad protein homolog smad2 Antibody, MADR2 Antibody, MADH2 Antibody, SMAD 2 Antibody, MAD homolog 2 Antibody, Mad-related protein 2 Antibody, JV18 Antibody, Mother against DPP homolog 2 Antibody, Mothers against DPP homolog 2 Antibody, Sma- and Mad-related protein 2 Antibody, SMAD family member 2 Antibody
SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.