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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SMAD2 Antibody (aa186‑273) LS‑C10213
SMAD2 antibody LS-C10213 is an unconjugated rabbit polyclonal antibody to SMAD2 from human, mouse and rat. Validated for WB.
Antibody
Catalog
Size
Price
LS-C10213-200
200 µl
$620

Popular SMAD2 Products

Anti-SMAD2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Anti-SMAD2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Anti-SMAD2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
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SMAD2 antibody. IHC(P): Human Breast Cancer Tissue.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, ICC, Western blot

Product Description

SMAD2 antibody LS-C10213 is an unconjugated rabbit polyclonal antibody to SMAD2 from human, mouse and rat. Validated for WB.

Specifications

Target
Human SMAD2
Synonyms
SMAD2, HMAD-2, HSMAD2, JV18-1, Mad protein homolog smad2, MADR2, MADH2, SMAD 2, MAD homolog 2, Mad-related protein 2, JV18, Mother against DPP homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD family member 2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:2000)
  • (applications tested for the base form of this product only)
Immunogen
SMAD2 antibody was raised against fusion protein corresponding to amino acids 186-273 of human Smad2.
Epitope
aa186-273
Specificity
Recognizes human Smad2 Mr 55-60kD and Smad3 Mr 50kD as determined by using Smad2 and Smad3 transfected Cos cells. Species cross-reactivity: Predicted to cross-react with mouse and rat based on conservation of immunogen sequence.
Usage
Suitable for use in Western Blot. Western Blot: 1:1000-1:2000 detects Smad2/3 in RIPA lysates of HepG2 cells. An additional unknown band was detected at Mr 97kD in the lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SMAD2
SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. Q15796 NM_005901 NP_005892.1


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Requested From: United States
Date Requested: 11/15/2018

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