Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (1:1000 - 1:2000)
- (applications tested for the base form of this product only)
SMAD2 antibody was raised against fusion protein corresponding to amino acids 186-273 of human Smad2.
Recognizes human Smad2 Mr 55-60kD and Smad3 Mr 50kD as determined by using Smad2 and Smad3 transfected Cos cells. Species cross-reactivity: Predicted to cross-react with mouse and rat based on conservation of immunogen sequence.
Suitable for use in Western Blot. Western Blot: 1:1000-1:2000 detects Smad2/3 in RIPA lysates of HepG2 cells. An additional unknown band was detected at Mr 97kD in the lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.