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Anti-SLIT3 Antibody (aa1164-1177) LS-C19113


Wt. Vol. Conc. Price
100 µg - 0.83 mg/ml Unavailable

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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SLIT3
Human, Mouse, Rat, Bovine, Dog
Western blot, ELISA


Human SLIT3
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:500 - 1:2000)
  • ELISA (1:2000 - 1:10000)

Specificity and Use

SLIT3 antibody was raised against synthetic peptide from human SLIT3.
aa 1164-1177 of Human SLIT-3 protein.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~168 kD corresponding to full length protein as well as multiple bands corresponding to isoforms of SLIT-3 by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About SLIT3

O75094 NM_003062 NP_003053.1

SLIT3 Antibody, KIAA0814 Antibody, MEGF5 Antibody, SLIL2 Antibody, Slit homolog 3 protein Antibody, Slit-3 Antibody, Slit (Drosophila) homolog 3 Antibody, Slit homolog 3 (Drosophila) Antibody

May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.

Western blot

Western blot
Anti-SLIT-3 Antibody - Western Blot. Western blot of Affinity Purified anti-SLIT-3 antibody shows detection of a predominant band at ~145 kD corresponding to SLIT-3 (arrowhead) in a bovine thyroid whole cell lysate using the 800 nm channel (green). ~35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MXHu ( for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (not shown). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: 
Date Requested: 4/23/2017

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