Target
Human SLC6A3 / Dopamine Transporter
Synonyms
SLC6A3 | DA transporter | DAT1 | DAT
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QLTSSTLTNPRQSP, from the internal region (near N-Terminus) of the protein sequence according to NP_001035.1.
Specificity
Human SLC6A3 / Dopamine Transporter.
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 85kD band observed in Human Brain (Substantia nigra) lysates (calculated MW of 68.5kD according to NP_001035.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mazei-Robison et al, J Neurosci. 2008 Jul 9;28(28):7040-6. PMID: 18614672). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SLC6A3 / Dopamine Transporter