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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SLC5A11 / SMIT2 Antibody (aa226‑275) LS‑C80636
SMIT2 antibody LS-C80636 is an unconjugated rabbit polyclonal antibody to SMIT2 (SLC5A11) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C80636-50
50 µg
$365

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Immunohistochemistry Image
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Product Description

SMIT2 antibody LS-C80636 is an unconjugated rabbit polyclonal antibody to SMIT2 (SLC5A11) from human, mouse, rat and other species. Validated for WB.
About SLC5A11 / SMIT2
Involved in the sodium-dependent cotransport of myo-inositol (MI) with a Na+:MI stoichiometry of 2:1. Exclusively responsible for apical MI transport and absorption in intestine. Also can transport D-chiro-inositol (DCI) but not L-fructose. Exhibits stereospecific cotransport of both D-glucose and D-xylose. May induce apoptosis through the TNF-alpha, PDCD1 pathway. Q8WWX8 NM_052944 NP_443176.2

SLC5A11 Antibody, Homolog of rabbit KST1 Antibody, KST1 Antibody, SLGTX Antibody, SMIT2 Antibody, RKST1 Antibody, SGLT6 Antibody


Specifications

Target
Human SLC5A11 / SMIT2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
SLC5A11 / SMIT2 antibody was raised against synthetic peptide located between aa226-275 of human SLC5A11 (Q8WWX8, NP_443176). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%); Opossum, Armadillo, Zebrafish (92%); Baboon, Platypus (91%); Sheep, Cat, Bat (84%); Shrew, Stickleback (83%); Trout, Salmon (81%).
Blocking Peptide
LS-E16199 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80636. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa226-275
Specificity
Human SLC5A11
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/24/2018

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