AE4 antibody LS-C11868 is an unconjugated rabbit polyclonal antibody to AE4 (SLC4A9) from human and rabbit. Validated for ELISA and WB.
Human SLC4A9 / AE4
(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
SLC4A9 / AE4 antibody was raised against a 15 AA Peptide sequence near the cytoplasmic C-terminus of human AE4 (1). The peptide was coupled to KLH.
The 15 AA human control peptide is 100% conserved in rabbit AE4. The sequence is also the same in proteins listed as human NBC5 (gene accession # AB032762, 657 aa) and NBC (gene accession # AF313465, 990 aa; XM_038736, 959 aa). No significant sequence homology of is detected with other AE1-3 or other transporters. Antibody cross-reactivity in various species is not known. Control peptide, because of its small size (<3kD), is not recommended for western. It should be used as a positive control in ELISA, dot blot or as blocking peptide (use 5-10 ug of control peptide per 1 ug IgG or 1 ul of antiserum) to confirm antibody specificity.
Suitable for use in ELISA and Western Blot.
PBS, 0.1% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
For research use only.