Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Immunofluorescence, Western blot (1:500 - 1:1000)
Specificity and Use
SLC2A10 / GLUT10 antibody was raised against a synthetic peptide containing residues 367-385 (ILSTAKKTKPHPRSGDPSA) of the human GLUT10 protein Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (95%).
Human GLUT10 protein.
Serum 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detected.
Cochlear sections from the organ of Corti.
NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detected using LS-C2788.
Cochlea from embryonic mice and mice up to 1 year old were removed and the pure membrane fraction loaded on 4-20% SDS gel, Following electrophoresis, transfer and blocking, the blot was incubated overnight at 4 degrees C with the GLUT10 primary antibody at a 1:500 dilution followed by incubation with secondary antibody at a 1:3000 dilution for 1 h at room temperature. Protein bands were visualized on x-ray film with SuperSignal west Femto chemiluminescent substrate. Lanes contain 10 ug of protein.
Requested From: United States
Date Requested: 9/25/2016