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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SKI Antibody (N‑Terminus) IHC‑plus™ LS‑B552 Best Seller
Note: This antibody replaces LS-C19444
SKI antibody LS-B552 is an unconjugated rabbit polyclonal antibody to human SKI. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B552-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

SKI antibody LS-B552 is an unconjugated rabbit polyclonal antibody to human SKI. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About SKI
May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. P12755 NM_003036 NP_003027.1

SKI Antibody, SKV Antibody, Proto-oncogene c-Ski Antibody, Ski oncogene Antibody, Ski oncoprotein Antibody, SGS Antibody


Specifications

Target
Human SKI
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E29930 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B552. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Usage
Immunohistochemistry: LS-B552 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B552 was determined to be 2.5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.
Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.

Immunohistochemistry

Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.
Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.
Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.

Western blot

Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.
Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.
Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.

Immunohistochemistry

Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.
Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.
Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.

Western blot

Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.
Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.
Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.

Immunohistochemistry

Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.
Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.
Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.

Western blot

Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.
Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.
Anti-SKI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.

Immunohistochemistry

Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.
Anti-SKI antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B552 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.
Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 ug/ml.

Western blot

Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.
Western blot of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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