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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIX6 Antibody (aa96‑124) LS‑C158976
SIX6 antibody LS-C158976 is an unconjugated rabbit polyclonal antibody to SIX6 from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C158976-400
400 µl
Unavailable

Popular SIX6 Products

Antibody
Species: Human
Applications: IHC, ELISA
SIX6 antibody LS-C29747 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence of HepG2 cells, using SIX6 Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunofluorescent analysis of SIX6 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Chicken
Applications: ICC, Immunofluorescence, Western blot
Western blot of HUVEC cell lysate using SIX6 Antibody
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

Product Description

SIX6 antibody LS-C158976 is an unconjugated rabbit polyclonal antibody to SIX6 from human and mouse. Validated for WB.
About SIX6
SIX6 is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in eye development. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2). O95475 NM_007374 NP_031400.2

SIX6 Antibody, Homeodomain protein OPTX2 Antibody, MCOPCT2 Antibody, Homeobox protein SIX6 Antibody, Six9 Antibody, Sine oculis homeobox homolog 6 Antibody, SIX homeobox 6 Antibody, Optic homeobox 2 Antibody, OPTX2 Antibody


Specifications

Target
Human SIX6
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E5318 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C158976. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa96-124
Specificity
This SIX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-124 amino acids from the Central region of human SIX6.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

SIX6 Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The SIX6 antibody detected the SIX6 protein (arrow).
SIX6 Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The SIX6 antibody detected the SIX6 protein (arrow).

Western blot

SIX6 Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The SIX6 antibody detected the SIX6 protein (arrow).
SIX6 Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The SIX6 antibody detected the SIX6 protein (arrow).

Western blot

SIX6 Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The SIX6 antibody detected the SIX6 protein (arrow).
SIX6 Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The SIX6 antibody detected the SIX6 protein (arrow).

Western blot

SIX6 Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The SIX6 antibody detected the SIX6 protein (arrow).
SIX6 Antibody western blot of mouse stomach tissue lysates (35 ug/lane). The SIX6 antibody detected the SIX6 protein (arrow).


Requested From: 
Date Requested: 6/20/2018

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