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Anti-SIX3 Antibody (C-Terminus) LS-C116055

Catalog	Size	Price
LS-C116055-50	50 µg	Unavailable

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Anti-SIX3 Antibody (aa138-166) LS-C325128

Antibody:	Rabbit Polyclonal (IgG) to Human SIX3
Reactivity:	Human
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Antibody:	Rabbit Polyclonal to Mouse SIX3
Reactivity:	Mouse, Human, Monkey, Rat, Bovine, Guinea pig
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

SIX3 antibody LS-C116055 Western blot of Mouse Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Mouse SIX3
Host:	Rabbit
Reactivity:	Mouse, Human, Monkey, Rat, Bovine, Guinea pig (tested or 100% immunogen sequence identity)
Predicted:	Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

SIX3 antibody was raised against synthetic peptide from C-Terminus of mouse Six3 (Q62233, NP_035511). Percent identity by BLAST analysis: Human, Chimpanzee, Galago, Marmoset, Mouse, Rat, Bovine, Opossum, Guinea pig (100%); Chicken, Xenopus, Zebrafish (92%).

Blocking Peptide:

LS-E13321 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116055. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

C-Terminus

Specificity:

Mouse SIX3

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About SIX3

Uniprot: O95343 NCBI: NM_005413 NP_005404.1

Synonyms: SIX3 Antibody, HPE2 Antibody, Sine oculis homeobox homolog 3 Antibody, SIX homeobox 3 Antibody, Homeobox protein SIX3 Antibody

Description: Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a ATTA homeodomain core recognition sequence on these target genes. During forebrain development represses WNT1 expression allowing zona limitans intrathalamica formation and thereby ensuring proper anterio-posterior patterning of the diencephalon and formation of the rostral diencephalon.

(More About SIX3)

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Western blot

SIX3 antibody LS-C116055 Western blot of Mouse Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/24/2018

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