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Anti-SIPR3 / EDG3 / S1P3 Antibody (aa12-25) IHC-plus™ LS-B2155

Note: This antibody replaces LS-C22994


Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Most Popular SIPR3 / EDG3 / S1P3 Antibodies

Anti-SIPR3 / EDG3 / S1P3 Antibody (N-Terminus) IHC-plus™ LS-A1752
Rabbit Polyclonal to Human SIPR3 / EDG3 / S1P3
IHC - Paraffin
Immunohistochemistry Image
Anti-SIPR3 / EDG3 / S1P3 Antibody (C-Terminus) IHC-plus™ LS-A1031
Rabbit Polyclonal to Human SIPR3 / EDG3 / S1P3
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Anti-SIPR3 / EDG3 / S1P3 Antibody IHC-plus™ LS-B511
Rabbit Polyclonal to Human SIPR3 / EDG3 / S1P3
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100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal (IgG) to Human SIPR3 / EDG3 / S1P3
IHC - Paraffin, ICC, Western blot


Human SIPR3 / EDG3 / S1P3
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Western blot (3 µg/ml)

Specificity and Use

SIPR3 / EDG3 / S1P3 antibody was raised against synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species sequence Homology: Mouse, rat: 79% (11/14). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (93%); Marmoset (86%).
Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.
Immunohistochemistry: LS-B2155 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2155 was determined to be 10 ug/ml.


TBS, pH 7.4, 5 mg/ml BSA
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (2)

Sphingosine 1-phosphate receptor 1 and 3 are upregulated in multiple sclerosis lesions. Ruben Van Doorn, Jack Van Horssen, Dennis Verzijl, Maarten Witte, Eric Ronken, Bert Van Het Hof, Kim Lakeman, Christine D. Dijkstra, Paul Van Der Valk, Arie Reijerkerk, Astrid E. Alewijnse, Stephan L. M. Peters, Helga E. De Vries. Glia. 2010 58:1465-76. (ICC, IHC-Fr; Human, Hamster) [Full Text Article] [PubMed:20648639]
Sphingosine-1-phosphate receptor 3 mediates sphingosine-1-phosphate induced release of weibel-palade bodies from endothelial cells. van Hooren KW, Spijkers LJ, van Breevoort D, Fernandez-Borja M, Bierings R, van Buul JD, Alewijnse AE, Peters SL, Voorberg J. PloS one. 2014 9:e91346. [Full Text Article] [PubMed:24632890] [PMC:PMC3954688]

About SIPR3 / EDG3 / S1P3

Q99500 NM_005226 NP_005217.2

S1PR3 Antibody, EDG-3 Antibody, EDG3 Antibody, LPB3 Antibody, S1P receptor EDG3 Antibody, S1P3 Antibody, S1P receptor Edg-3 Antibody, S1P receptor 3 Antibody

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.


Anti-EDG3 antibody IHC of human prostate, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2155 concentration 10 ug/ml.

Requested From: 
Date Requested: 3/29/2017

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