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Anti-SIK2 / SNF1LK2 Antibody (aa100-150) IHC-plus™ LS-B2068

Note: This antibody replaces LS-C19398, LS-C91389

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SIK2 / SNF1LK2 Antibodies

Anti-SIK2 / SNF1LK2 Antibody (clone S15G10) IHC-plus™ LS-B1898
Mouse Monoclonal [clone S15G10] (IgG1) to Human SIK2 / SNF1LK2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-SIK2 / SNF1LK2 Antibody (aa100-150) IHC-plus™ LS-B2068
Rabbit Polyclonal to Human SIK2 / SNF1LK2
Human, Mouse, Rat, Bovine, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SIK2 / SNF1LK2 Antibody (Internal) IHC-plus™ LS-B7103
Rabbit Polyclonal to Human SIK2 / SNF1LK2
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SIK2 / SNF1LK2
Human, Mouse, Rat, Bovine, Dog
IHC - Paraffin, Western blot
Unconjugated

Details

Human SIK2 / SNF1LK2
Rabbit
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

SIK2 / SNF1LK2 antibody was raised against a portion of amino acids 100-150 of human SIK2.
aa100-150
Human SIK2 is a 926 amino acid protein. The approximately 120 kD band is higher than the theoretical expected size, but this band, as well as the approximately 25 kD lower band, are blocked by immunizing peptide.
Immunohistochemistry: LS-B2068 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2068 was determined to be 10 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About SIK2 / SNF1LK2

Q9H0K1 AB018324 BAA34501.3

SIK2 Antibody, KIAA0781 Antibody, LOH11CR1I Antibody, SNF1LK2 Antibody, QIK Antibody, Qin-induced kinase Antibody, Salt-inducible kinase 2 Antibody, SIK-2 Antibody, SNF1-like kinase 2 Antibody

Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.

Immunohistochemistry

Immunohistochemistry
Anti-SIK2 / SNF1LK2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2068 concentration 10 ug/ml.

Western blot

Western blot
Western blot of SIK2 in human brain in the A) absence and B) presence of immunizing peptide, using antibody at 2 ug/ml.

Requested From: United States
Date Requested: 12/7/2016

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