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Anti-SIGLEC5 / CD170 Antibody (aa17-434) LS-C150060

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SIGLEC5 / CD170 Antibodies

Anti-SIGLEC5 / CD170 Antibody (aa8-39) LS-C97825
Rabbit Polyclonal to Human SIGLEC5 / CD170
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SIGLEC5 / CD170 Antibody (C-Terminus, AP) LS-C220650
Rabbit Polyclonal (IgG) to Human SIGLEC5 / CD170
Human, Mouse
Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human SIGLEC5 / CD170
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human SIGLEC5 / CD170
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Flow Cytometry

Specificity and Use

SIGLEC5 / CD170 antibody was raised against mouse myeloma cell line NS0-derived recombinant human Siglec5 Glu17-Thr434 Accession # O15389.
aa17-434
Detects human Siglec5/14 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human.
Western Blot, 0.1 µg/ml, Recombinant Human Siglec-5 Fc Chimera. Flow Cytometry, 2.5 µg/106 cells. Immunohistochemistry, 5-15 µg/ml, Immersion fixed paraffin-embedded sections of human lymph node.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About SIGLEC5 / CD170

O15389 NM_003830 NP_003821.1

SIGLEC5 Antibody, CD170 antigen Antibody, CD170 Antibody, CD33 antigen-like 2 Antibody, OB-binding protein 2 Antibody, SIGLEC-5 Antibody, CD33L2 Antibody, OB-BP2 Antibody, OBBP2 Antibody, Obesity-binding protein 2 Antibody

SIGLEC5 / CD170 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils.

Flow Cytometry

Flow Cytometry
Detection of Siglec-5/14 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Human Siglec-5/14 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.

Neutralization

Neutralization
Cell Adhesion Mediated by Siglec-5 and Neutralization by Human Siglec-5/14 Antibody. Recombinant Human Siglec-5 Fc Chimera, immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 ug/ml) is neutralized (green line) by increasing concentrations of Human Siglec-5/14 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.5-2.0 ug/ml.

Requested From: United States
Date Requested: 12/9/2016

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