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Anti-Sialylated Mucin-Type-O-Glycans Antibody (clone FW17) LS-C132098


Wt. Vol. Conc. Price
10 µg 10 µl 1 mg/ml Unavailable
100 µg - - Unavailable

Most Popular Sialylated Mucin-Type-O-Glycans Antibodies

Anti-Sialylated Mucin-Type-O-Glycans Antibody (clone FW17) LS-C132098
Mouse Monoclonal [clone FW17] (IgM) to Human Sialylated Mucin-Type-O-Glycans
IHC - Paraffin, ICC, ELISA

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone FW17] (IgM) to Human Sialylated Mucin-Type-O-Glycans
IHC - Paraffin, ICC, ELISA


Human Sialylated Mucin-Type-O-Glycans
Human (tested or 100% immunogen sequence identity)
IgM Monoclonal [FW17]


  • IHC - Paraffin
  • ICC

Specificity and Use

Purified mucus-glycoproteins from human amniotic fluid.
Sialylated Mucin-Type-O-Glycans.
Tumor and normal tissues were first fixed in 6% neutral phosphate-buffered formalin and dehydrated in ethanol by standard histological techniques. After embedding all tissues in paraffin, serial microtome tissue sections (5 µm) were used for comparative immunohistological studies. Reactivity of hybridoma tissue culture supernatants was determined using a four-step immunoperoxidase technique. After deparaffinization in xylene, rehydration through graded ethanol, and blocking endogenous peroxidase activity with 2% H202 in absolute methanol for 30 min, the sections were incubated as follows: (1) normal swine serum (5% in PBS), (2) MAb in tissue culture supernatant or mouse ascites fluid diluted to 1/100 in PBS, (3) rabbit anti-mouse immunoglobulin (2% in PBS), (4) swine anti-rabbit immunoglobulin (2% in PBS), (5) rabbit peroxidase anti-peroxidase (PAP) complex. The slides were treated with 0.2 mg/ml - ' 3-amino-9-ethylcarbazole in 0.02 M sodium acetate buffer containing 0.01% H202, then counterstained with hematoxylin, and mounted in glycerol jelly. All incubation steps were performed in a moist chamber at 21°C for 30 min.


Lyophilized from PBS, pH 7.4
10 µl Distilled water
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C.
For research use only.

About Sialylated Mucin-Type-O-Glycans

Requested From: 
Date Requested: 3/28/2017

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