Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone FW17] (IgM) to Human Sialylated Mucin-Type-O-Glycans
IHC - Paraffin, ICC, ELISA
Human Sialylated Mucin-Type-O-Glycans
Human (tested or 100% immunogen sequence identity)
IgM Monoclonal [FW17]
IHC - Paraffin
Specificity and Use
Purified mucus-glycoproteins from human amniotic fluid.
Tumor and normal tissues were first fixed in 6% neutral phosphate-buffered formalin and dehydrated in ethanol by standard histological techniques. After embedding all tissues in paraffin, serial microtome tissue sections (5 µm) were used for comparative immunohistological studies. Reactivity of hybridoma tissue culture supernatants was determined using a four-step immunoperoxidase technique. After deparaffinization in xylene, rehydration through graded ethanol, and blocking endogenous peroxidase activity with 2% H202 in absolute methanol for 30 min, the sections were incubated as follows: (1) normal swine serum (5% in PBS), (2) MAb in tissue culture supernatant or mouse ascites fluid diluted to 1/100 in PBS, (3) rabbit anti-mouse immunoglobulin (2% in PBS), (4) swine anti-rabbit immunoglobulin (2% in PBS), (5) rabbit peroxidase anti-peroxidase (PAP) complex. The slides were treated with 0.2 mg/ml - ' 3-amino-9-ethylcarbazole in 0.02 M sodium acetate buffer containing 0.01% H202, then counterstained with hematoxylin, and mounted in glycerol jelly. All incubation steps were performed in a moist chamber at 21°C for 30 min.
Lyophilized from PBS, pH 7.4
10 µl Distilled water
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C.