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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SIAH2 Antibody (aa216‑244) LS‑C161085
SIAH2 antibody LS-C161085 is an unconjugated rabbit polyclonal antibody to human SIAH2. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C161085-400
400 µl
Unavailable

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Product Description

SIAH2 antibody LS-C161085 is an unconjugated rabbit polyclonal antibody to human SIAH2. Validated for Flow, IHC and WB.
About SIAH2
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. O43255 NM_005067 NP_005058.3

SIAH2 Antibody, HSiah2 Antibody, Seven in absentia homolog 2 Antibody, Siah-2 Antibody


Specifications

Target
Human SIAH2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8024 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C161085. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa216-244
Specificity
This SIAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-244 amino acids from the Central region of human SIAH2.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Customer Reviews (0)


Images


Immunohistochemistry

SIAH2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIAH2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

SIAH2 Antibody western blot of T47D cell line lysates (35 ug/lane). The SIAH2 antibody detected the SIAH2 protein (arrow).
SIAH2 Antibody western blot of T47D cell line lysates (35 ug/lane). The SIAH2 antibody detected the SIAH2 protein (arrow).

Flow Cytometry

SIAH2 Antibody flow cytometry of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SIAH2 Antibody flow cytometry of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SIAH2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIAH2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

SIAH2 Antibody western blot of T47D cell line lysates (35 ug/lane). The SIAH2 antibody detected the SIAH2 protein (arrow).
SIAH2 Antibody western blot of T47D cell line lysates (35 ug/lane). The SIAH2 antibody detected the SIAH2 protein (arrow).

Flow Cytometry

SIAH2 Antibody flow cytometry of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SIAH2 Antibody flow cytometry of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SIAH2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIAH2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

SIAH2 Antibody western blot of T47D cell line lysates (35 ug/lane). The SIAH2 antibody detected the SIAH2 protein (arrow).
SIAH2 Antibody western blot of T47D cell line lysates (35 ug/lane). The SIAH2 antibody detected the SIAH2 protein (arrow).

Flow Cytometry

SIAH2 Antibody flow cytometry of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SIAH2 Antibody flow cytometry of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

SIAH2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
SIAH2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

SIAH2 Antibody western blot of T47D cell line lysates (35 ug/lane). The SIAH2 antibody detected the SIAH2 protein (arrow).
SIAH2 Antibody western blot of T47D cell line lysates (35 ug/lane). The SIAH2 antibody detected the SIAH2 protein (arrow).

Flow Cytometry

SIAH2 Antibody flow cytometry of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SIAH2 Antibody flow cytometry of T47D cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/22/2018

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