(tested or 100% immunogen sequence identity)
SHPRH antibody was raised against residues 515-535 (DKTKKQAVGSPRKIEKELRKS) of the mouse SHPRH protein. 95% sequence identity to rat. 90% sequence identity to human Percent identity by BLAST analysis: Mouse (100%); Rat (95%); Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Panda, Dog, Pig (90%); Bat (86%); Marmoset, Bovine, Horse, Rabbit (81%).
Immunohistochemistry: LS-B308 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B308 was determined to be 2.5 ug/ml.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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