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Anti-SHPRH Antibody (aa515-535) IHC-plus™ LS-B308

Note: This antibody replaces LS-C2894, LS-C10149

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml Unavailable

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18 SHPRH Antibodies

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Anti-SHPRH Antibody (aa515-535) IHC-plus™ LS-B308
Rabbit Polyclonal to Mouse SHPRH
Mouse
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-SHPRH Antibody (clone 1H3) LS-C337139
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Western blot Image
Anti-SHPRH Antibody (clone 7B2) LS-C337140
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Anti-SHPRH Antibody (clone 5G8) LS-C337141
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Western blot Image
Anti-SHPRH Antibody (clone 2994C1a) LS-C342764
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Rabbit Polyclonal to Mouse SHPRH
Mouse
IHC - Paraffin
Unconjugated

Details

Mouse SHPRH
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

IHC - Paraffin (2.5 µg/ml)

Specificity and Use

SHPRH antibody was raised against residues 515-535 (DKTKKQAVGSPRKIEKELRKS) of the mouse SHPRH protein. 95% sequence identity to rat. 90% sequence identity to human Percent identity by BLAST analysis: Mouse (100%); Rat (95%); Human, Gorilla, Gibbon, Monkey, Hamster, Elephant, Panda, Dog, Pig (90%); Bat (86%); Marmoset, Bovine, Horse, Rabbit (81%).
aa515-535
SHPRH.
Immunohistochemistry: LS-B308 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B308 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About SHPRH

Q149N8 AA476814 NP_775105.1

SHPRH Antibody, BA545I5.2 Antibody, KIAA2023 Antibody

E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.

Immunohistochemistry

Immunohistochemistry
Anti-SHPRH antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B308 concentration 5 ug/ml.

Requested From: 
Date Requested: 4/26/2017

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