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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SHFM3 / FBXW4 Antibody (aa157‑171) IHC‑plus™ LS‑B804 Best Seller
Note: This antibody replaces LS-C3218
FBXW4 antibody LS-B804 is an unconjugated rabbit polyclonal antibody to human FBXW4 (SHFM3). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B804-50
50 µg (1 mg/ml)
Unavailable

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Product Description

FBXW4 antibody LS-B804 is an unconjugated rabbit polyclonal antibody to human FBXW4 (SHFM3). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
About SHFM3 / FBXW4
SHFM3 / FBXW4 is a member of the F-box/WD-40 gene family, which recruit specific target proteins through their WD-40 protein-protein binding domains for ubiquitin mediated degradation. In mouse, a highly similar protein is thought to be responsible for maintaining the apical ectodermal ridge of developing limb buds; disruption of the mouse gene results in the absence of central digits, underdeveloped or absent metacarpal/metatarsal bones and syndactyly. P57775 NM_022039 NP_071322.1

FBXW4 Antibody, DAC Antibody, Dactylin Antibody, FBW4 Antibody, FBWD4 Antibody, F-box/WD repeat protein 4 Antibody, SHFM3 Antibody, SHSF3 Antibody


Specifications

Target
Human SHFM3 / FBXW4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ELISA (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SHFM3 / FBXW4 antibody was raised against synthetic peptide from human FBXW4 / SHFM3.
Epitope
aa157-171
Specificity
Amino acids 157 to 171 of human FBXW4(SHFM3)
Usage
Immunohistochemistry: LS-B804 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B804 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-FBXW4 / SHFM3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B804 concentration 10 ug/ml.
Anti-FBXW4 / SHFM3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B804 concentration 10 ug/ml.

Immunohistochemistry

Anti-FBXW4 / SHFM3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B804 concentration 10 ug/ml.
Anti-FBXW4 / SHFM3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B804 concentration 10 ug/ml.

Immunohistochemistry

Anti-FBXW4 / SHFM3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B804 concentration 10 ug/ml.
Anti-FBXW4 / SHFM3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B804 concentration 10 ug/ml.

Immunohistochemistry

Anti-FBXW4 / SHFM3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B804 concentration 10 ug/ml.
Anti-FBXW4 / SHFM3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B804 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/19/2018

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