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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SH2B3 / LNK Antibody (aa2‑11) LS‑C55071
Note: This antibody replaces LS-C71767
LNK antibody LS-C55071 is an unconjugated goat polyclonal antibody to human LNK (SH2B3). Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C55071-100
100 µg
$405
Description
LNK antibody LS-C55071 is an unconjugated goat polyclonal antibody to human LNK (SH2B3). Validated for Peptide-ELISA and WB.
Target
Human SH2B3 / LNK
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:64000) & (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Failed Applications
  • IHC - Paraffin
Immunogen
SH2B3 / LNK antibody was raised against synthetic peptide NGPALQPSSPC from the N-terminus of human SH2B3 (NP_005466.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (90%), Monkey (90%), Marmoset (90%), Panda (80%), Dog (80%), Rabbit (80%), Pig (80%).
Blocking Peptide
LS-E25756 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55071. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-11
Specificity
Human SH2B3.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SH2B3 / LNK
Q9UQQ2 NM_005475 NP_005466.1

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Images

Western blot

HEK293 overexpressing SH2B3 (RC218359) and probed with (mock transfection in first lane).
HEK293 overexpressing SH2B3 (RC218359) and probed with (mock transfection in first lane).

Western blot

Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SH2B3 (RC218359) and probed with (mock transfection in first lane).
HEK293 overexpressing SH2B3 (RC218359) and probed with (mock transfection in first lane).

Western blot

Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SH2B3 (RC218359) and probed with (mock transfection in first lane).
HEK293 overexpressing SH2B3 (RC218359) and probed with (mock transfection in first lane).

Western blot

Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing SH2B3 (RC218359) and probed with (mock transfection in first lane).
HEK293 overexpressing SH2B3 (RC218359) and probed with (mock transfection in first lane).

Western blot

Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.3 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/11/2018
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