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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SGCG / Gamma‑Sarcoglycan Antibody (aa151‑200) LS‑C80650
Gamma-Sarcoglycan antibody LS-C80650 is an unconjugated rabbit polyclonal antibody to Gamma-Sarcoglycan (SGCG) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C80650-100
100 µg
Unavailable

Popular SGCG / Gamma-Sarcoglycan Products

Immunoprecipitation of SGCG transfected lysate using anti-SGCG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SGCG rabbit polyclonal antibody.
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
SGCG Antibody western blot of MCF-7,MDA-MB231,MDA-MB453 cell line lysates (35 ug/lane). The SGCG antibody detected the SGCG protein (arrow).
Species: Human
Applications: Western blot
Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Applications: Western blot
Western blot analysis of extracts of various cells.
Species: Human
Applications: Western blot
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

Gamma-Sarcoglycan antibody LS-C80650 is an unconjugated rabbit polyclonal antibody to Gamma-Sarcoglycan (SGCG) from human, mouse, rat and other species. Validated for WB.
About SGCG / Gamma-Sarcoglycan
SGCG / Gamma-Sarcoglycan is gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Q13326 NM_000231 NP_000222.1

SGCG Antibody, 35DAG Antibody, DMDA Antibody, DMDA1 Antibody, Gamma sarcoglycan Antibody, Gamma-sarcoglycan Antibody, LGMD2C Antibody, MAM Antibody, DAGA4 Antibody, Gamma-SG Antibody, SCARMD2 Antibody


Specifications

Target
Human SGCG / Gamma-Sarcoglycan
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Hamster, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa151-200 of human SGCG (Q13326, NP_000222). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Zebrafish (100%); Hamster, Lizard, Xenopus, Stickleback (92%).
Blocking Peptide
LS-E16250 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80650. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa151-200
Specificity
Human SGCG
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal heart cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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