(tested or 100% immunogen sequence identity)
SFRP5 antibody was raised against n/a
Reacts with residues 25-38 [APARCEEYDYYGWQ] of human SFRP5. 92% identity bovine. 85% identity rat. 78% identity mouse.
Immunohistochemistry: LS-B307 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B307 was determined to be 2.5 ug/ml.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.