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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SFN / Stratifin / 14‑3‑3 Sigma Antibody (clone 3C3) IHC‑plus™ LS‑B4334
Note: This antibody replaces LS-C104959
14-3-3 Sigma antibody LS-B4334 is an unconjugated mouse monoclonal antibody to human 14-3-3 Sigma (SFN / Stratifin). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4334-50
50 µg
Unavailable

Popular SFN / Stratifin / 14-3-3 Sigma Products

Formalin-fixed, paraffin-embedded human skin stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis. This image was taken for the unmodified form of this product. Other forms have not been tested.
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Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3281 dilution 1:250.
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Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5855 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Product Description

14-3-3 Sigma antibody LS-B4334 is an unconjugated mouse monoclonal antibody to human 14-3-3 Sigma (SFN / Stratifin). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About SFN / Stratifin / 14-3-3 Sigma
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. P31947 NM_006142 NP_006133.1

SFN Antibody, 14-3-3 sigma Antibody, 14-3-3 protein sigma Antibody, HME1 Antibody, Stratifin Antibody, YWHAS Antibody


Specifications

Target
Human SFN / Stratifin / 14-3-3 Sigma
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [3C3]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SFN (AAH00329, 1 a.a. ~ 249 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western Blot (Cell lysate) - positive control HeLa.
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Western blot

SFN monoclonal antibody, clone 3C3 Western blot of SFN expression in HeLa.
SFN monoclonal antibody, clone 3C3 Western blot of SFN expression in HeLa.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Western blot

SFN monoclonal antibody, clone 3C3 Western blot of SFN expression in HeLa.
SFN monoclonal antibody, clone 3C3 Western blot of SFN expression in HeLa.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Western blot

SFN monoclonal antibody, clone 3C3 Western blot of SFN expression in HeLa.
SFN monoclonal antibody, clone 3C3 Western blot of SFN expression in HeLa.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Immunohistochemistry

Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.
Anti-SFN / 14-3-3 Sigma antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4334 concentration 5 ug/ml.

Western blot

SFN monoclonal antibody, clone 3C3 Western blot of SFN expression in HeLa.
SFN monoclonal antibody, clone 3C3 Western blot of SFN expression in HeLa.


Requested From: 
Date Requested: 6/18/2018

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