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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SET / TAF‑I Antibody (aa66‑81) IHC‑plus™ LS‑B2058
Note: This antibody replaces LS-C727
TAF-I antibody LS-B2058 is an unconjugated rabbit polyclonal antibody to TAF-I (SET) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2058-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

TAF-I antibody LS-B2058 is an unconjugated rabbit polyclonal antibody to TAF-I (SET) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About SET / TAF-I
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Q01105 NM_003011 NP_003002.2

SET Antibody, 2PP2A Antibody, HLA-DR-associated protein II Antibody, I-2PP2A Antibody, TAF-IBETA Antibody, SET nuclear oncogene Antibody, TAF-I Antibody, Template-activating factor I Antibody, I2PP2A Antibody, IGAAD Antibody, IPP2A2 Antibody, PHAPII Antibody, Protein SET Antibody


Specifications

Target
Human SET / TAF-I
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SET / TAF-I antibody was raised against synthetic peptides of amino acids 66-81 (PFFQKRSELIAKIPNF) and 135-151 (ENKVLSKEFHLNESGDP) of human INHAT-1. Sequence homology is 100% in mouse and rat. Percent identity by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%); Gorilla, Stickleback (94%); Dog (88%).
Epitope
aa66-81
Specificity
An approx. 37 kD band is observed.
Usage
Immunohistochemistry: LS-B2058 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2058 was determined to be 10 ug/ml.
Presentation
PBS, 0.02% sodium azide, 0.1 mg/ml BSA. sodium azide is toxic.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.
Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.

Western blot

Western blot of INHAT-1 using antibody against Jurkat cell lysate.
Western blot of INHAT-1 using antibody against Jurkat cell lysate.

Immunohistochemistry

Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.
Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.

Western blot

Western blot of INHAT-1 using antibody against Jurkat cell lysate.
Western blot of INHAT-1 using antibody against Jurkat cell lysate.

Immunohistochemistry

Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.
Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.

Western blot

Western blot of INHAT-1 using antibody against Jurkat cell lysate.
Western blot of INHAT-1 using antibody against Jurkat cell lysate.

Immunohistochemistry

Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.
Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.

Western blot

Western blot of INHAT-1 using antibody against Jurkat cell lysate.
Western blot of INHAT-1 using antibody against Jurkat cell lysate.


Requested From: 
Date Requested: 6/19/2018

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