Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (10 µg/ml)
- Western blot (1 - 2 µg/ml)
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SET / TAF-I antibody was raised against synthetic peptides of amino acids 66-81 (PFFQKRSELIAKIPNF) and 135-151 (ENKVLSKEFHLNESGDP) of human INHAT-1. Sequence homology is 100% in mouse and rat. Percent identity by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%); Gorilla, Stickleback (94%); Dog (88%).
An approx. 37 kD band is observed.
Immunohistochemistry: LS-B2058 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2058 was determined to be 10 ug/ml.
PBS, 0.02% sodium azide, 0.1 mg/ml BSA. sodium azide is toxic.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.