Human SERPINB9 / PI9

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Pig

IgG1 Monoclonal

7D8

Unconjugated

Protein G purified

Unmodified

Recombinant PI-9 produced in P. pastoris.

Specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kD intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone Detects a band of approximately 42kD under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence.

• IHC
• IHC - Paraffin (1:50)
• Western blot
• Flow Cytometry (1:10 - 1:100)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required for this application. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Tonsil.

PBS, 0.05% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P50453 NCBI: NM_004155 NP_004146.1