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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SEMA4D / Semaphorin 4D / CD100 Antibody LS‑C43410
Note: This antibody replaces LS-C57269, LS-C43408
Antibody
CD100 antibody LS-C43410 is an unconjugated mouse monoclonal antibody to CD100 (SEMA4D / Semaphorin 4D) from human, cynomolgus monkey and rhesus monkey. Validated for Flow, IP and WB.
Catalog
Size
Price
LS-C43410-100
100 µg (1 mg/ml)
Unavailable

Popular SEMA4D / Semaphorin 4D / CD100 Products

Antibody
Species: Human
Applications: IHC, IHC - Frozen, Flow Cytometry
Peripheral Human lymphocytes stained with Mouse anti Human CD100: APC. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Cynomolgus monkey, Rhesus monkey
Applications: Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Flow Cytometry
Immunohistochemistry of formalin-fixed, paraffin-embedded kidney transplant tissue (40X) stained with Complement 4d antibody (1:200), peroxidase-conjugate and DAB chromogen.
Species: Human
Applications: IHC, IHC - Paraffin

Product Description

CD100 antibody LS-C43410 is an unconjugated mouse monoclonal antibody to CD100 (SEMA4D / Semaphorin 4D) from human, cynomolgus monkey and rhesus monkey. Validated for Flow, IP and WB.
About SEMA4D / Semaphorin 4D / CD100
Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Q92854 NM_006378 NP_006369.3

SEMA4D Antibody, A8 Antibody, BB18 Antibody, C9orf164 Antibody, CD100 antigen Antibody, Coll-4 Antibody, GR3 Antibody, Semaphorin-4D Antibody, M-sema-G Antibody, SEMAJ Antibody, CD100 Antibody


Specifications

Target
Human SEMA4D / Semaphorin 4D / CD100
Host
Mouse
Reactivity
Human, Cynomolgus monkey, Rhesus monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with FITC, RPE, APC.
Purification
Purified
Modifications
Unmodified. Also available Low Endotoxin.
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:1)
  • (applications tested for the base form of this product only)
Immunogen
Human PHA activated lymphocytes.
Specificity
Recognizes human Sepaphorin 4D, also known as CD100, A8, BB18 or GR3. CD100 is a 841 amino acid ~150 kD homodimeric single pass type 1 transmembrane cell surface glycoprotein actin as a receptor for some members of the plexin family. CD100 is expressed at low levels by peripheral blood T cells, and at higher density on activated T cells and B cells, it is also expresses at high levels in fetal kidney with lower levels in fetal brain, liver and lung.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, pH 7.4, 0.09% sodium azide.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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