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Anti-SEMA4D / Semaphorin 4D / CD100 Antibody (aa24-657) LS-C150246

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SEMA4D / Semaphorin 4D / CD100 Antibodies

Anti-SEMA4D / Semaphorin 4D / CD100 Antibody (clone 133-1C6, Biotin) LS-C134691
Mouse Monoclonal [clone 133-1C6] (IgM) to Human SEMA4D / Semaphorin 4D / CD100
Human
Flow Cytometry
Biotin Conjugated
Flow Cytometry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Mouse SEMA4D / Semaphorin 4D / CD100
Mouse
IHC - Frozen, Western blot, Flow Cytometry
Unconjugated

Details

Mouse SEMA4D / Semaphorin 4D / CD100
Sheep
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Frozen (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Flow Cytometry

Specificity and Use

SEMA4D / Semaphorin 4D / CD100 antibody was raised against mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100 Phe24-Thr657 Accession # NP_038688.
aa24-657
Detects mouse Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity.
Western Blot, 0.1 µg/ml, Recombinant Mouse Semaphorin 4D/CD100. Flow Cytometry, 2.5 µg/106 cells. Immunohistochemistry, 5-15 µg/ml, Immersion fixed frozen sections of mouse embryonic eye cup (15 d. p. c.).

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About SEMA4D / Semaphorin 4D / CD100

Q92854 NM_006378 NP_006369.3

SEMA4D Antibody, A8 Antibody, BB18 Antibody, C9orf164 Antibody, CD100 antigen Antibody, Coll-4 Antibody, GR3 Antibody, Semaphorin-4D Antibody, M-sema-G Antibody, SEMAJ Antibody, CD100 Antibody

Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro).

Flow Cytometry

Flow Cytometry
Detection of Semaphorin 4D/ CD100 in EL4 Mouse Cell Line by Flow Cytometry. EL4 mouse lymphoblast cell line were stained with Sheep Anti-Mouse Semaphorin 4D/CD100 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by NorthernLights 637-conjugated Anti-Sheep IgG Secondary Antibody.
 

Requested From: United States
Date Requested: 12/4/2016

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