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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SEMA4A / Semaphorin 4A Antibody LS‑C126255
Antibody
Semaphorin 4A antibody LS-C126255 is an unconjugated mouse polyclonal antibody to human Semaphorin 4A (SEMA4A). Validated for ELISA and WB.
Catalog
Size
Price
LS-C126255-50
50 µl
Unavailable

Popular SEMA4A / Semaphorin 4A Products

IHC of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of SEMA4A (N532) pAb in paraffin-embedded human brain tissue.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: Flow Cytometry
Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Monkey
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Western blot of SEMA4A antibody
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

Semaphorin 4A antibody LS-C126255 is an unconjugated mouse polyclonal antibody to human Semaphorin 4A (SEMA4A). Validated for ELISA and WB.
About SEMA4A / Semaphorin 4A
SEMA4A / Semaphorin 4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Q9H3S1 NM_022367 NP_071762.2

SEMA4A Antibody, CORD10 Antibody, RP11-54H19.2 Antibody, RP35 Antibody, Sema B Antibody, Semaphorin-4A Antibody, Semaphorin-B Antibody, SEMB Antibody, SEMAB Antibody


Specifications

Target
Human SEMA4A / Semaphorin 4A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
Partial recombinant human Semaphorin 4A (33-132) with a GST tag.
Specificity
Recognizes human Semaphorin 4A.
Usage
Suitable for use in ELISA and Western Blot.
Presentation
50% glycerol
Storage
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least1 year at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/21/2018

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