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Anti-SELPLG / PSGL-1 / CD162 Antibody (aa51-63, Biotin) IHC-plus™ LS-B2507

Note: This antibody replaces LS-C35859
Catalog Size Price
LS-B2507-50 50 µg Unavailable

Most Popular SELPLG / PSGL-1 / CD162 Antibodies

Anti-SELPLG / PSGL-1 / CD162 Antibody (clone HECA-452) IHC-plus™ LS-B3879
Rat Monoclonal [clone HECA-452] (IgM) to Human SELPLG / PSGL-1 / CD162
Human, Mouse
IHC - Paraffin, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-SELPLG / PSGL-1 / CD162 Antibody (clone TC2) IHC-plus™ LS-B4514
Mouse Monoclonal [clone TC2] (IgG1) to Human SELPLG / PSGL-1 / CD162
Human
IHC - Paraffin, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-SELPLG / PSGL-1 / CD162 Antibody (clone PL1/3E2-25-5, Biotin) LS-C134781
Mouse Monoclonal [clone PL1/3E2-25-5] (IgG1) to Human SELPLG / PSGL-1 / CD162
Human
Western blot, Flow Cytometry, Functional Assay
Biotin Conjugated
Flow Cytometry Image
Anti-SELPLG / PSGL-1 / CD162 Antibody (clone PL1/3E2-25-5, Azide-free) LS-C134783
Mouse Monoclonal [clone PL1/3E2-25-5] (IgG1) to Human SELPLG / PSGL-1 / CD162
Human
Western blot, Flow Cytometry, Functional Assay
Unconjugated, Azide-free
Anti-SELPLG / PSGL-1 / CD162 Antibody LS-C183429
Rabbit Polyclonal (IgG) to Mouse SELPLG / PSGL-1 / CD162
Mouse, Human, Rat
IHC, Western blot
Unconjugated

100% Guaranteed
Mouse Monoclonal (IgG1) to Human SELPLG / PSGL-1 / CD162
Human
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Biotin Conjugated

Details

Human SELPLG / PSGL-1 / CD162
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Biotin
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen
  • Western blot
  • Flow Cytometry

Specificity and Use

Purified (With Antibiotics) human neutrophil PSGL-1.
aa51-63
Recognizes human CD162 molecule at 120kD within a region containing aa51-63.
Immunohistochemistry: LS-B2507 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2507 was determined to be 5 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

FBS, 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide.
Short term: +4°C, Long term: aliquot to avoid repeated freez/thaw cycles, and store at -20°C.
For research use only.

About SELPLG / PSGL-1 / CD162

Q14242 NM_003006 NP_002997.2

SELPLG Antibody, CD162 antigen Antibody, CLA Antibody, CD162 Antibody, PSGL1 Antibody, PSGL-1 Antibody, Selectin P ligand Antibody

SELPLG / PSGL-1 / CD162 is a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins.

Immunohistochemistry

Immunohistochemistry
Anti-SELPLG / CD162 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2507 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 10/20/2017

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