Target
Human Secretory Component
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Biotin.
Also available
Unconjugated or conjugated with
FITC,
HRP,
TRITC.
Purification
Purified IgG
Immunogen
Human secretory component (free and bound)
Specificity
Human Secretory Component. The reactivity of the antiserum is restricted to secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. Cross-reactivity The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Applications
- Immunofluorescence
- Western blot
- ELISA
- Applications tested for the base form of this product only
Usage
This product is intended for use to identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:25 upwards; in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with 0.5ml distilled H2O.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee