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Anti-SDHB Antibody (aa271-280) IHC-plus™ LS-B2872

Note: This antibody replaces LS-C55442

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SDHB Antibodies

Anti-SDHB Antibody (aa271-280) IHC-plus™ LS-B2872
Goat Polyclonal to Human SDHB
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SDHB Antibody (aa1-150) IHC-plus™ LS-B6428
Rabbit Polyclonal to Human SDHB
Human, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SDHB Antibody (aa205-234, FITC) LS-C285281
Rabbit Polyclonal (IgG) to Human SDHB
Human
Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human SDHB
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human SDHB
Goat
Human (tested or 100% immunogen sequence identity)
Monkey, Dog, Horse, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • ELISA (1:8000)

Specificity and Use

SDHB antibody was raised against synthetic peptide C-ATYKEKKASV from the C-terminus of human SDHB (NP_002991.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Dog, Horse, Pig, Trout, Pufferfish (90%); Bovine, Catfish, Sablefish (80%).
LS-E25921 - Lyophilized - 100 µg - $145.00
aa271-280
Human SDHB.
Immunohistochemistry: LS-B2872 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2872 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SDHB

P21912 NM_003000 NP_002991.2

SDHB Antibody, SDH1 Antibody, SDHIP Antibody, SDH Antibody, IP Antibody, PGL4 Antibody, SDH2 Antibody

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme.

Immunohistochemistry

Immunohistochemistry
Anti-SDHB antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2872 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.03 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/8/2016

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