Target
Human SCP2 / SCPX
Synonyms
SCP2 | NLTP | NSL-TP | SCP-CHI | SCP-X | Sterol carrier protein 2 | Sterol carrier protein X | SCP-2 | SCPX
Reactivity
Human, Monkey, Rat, Bat, Bovine, Hamster, Horse, Rabbit, Drosophila
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KNHKHSVNNPYSQ, from the internal region of the protein sequence according to NP_001007099.1NP_002970.2NP_001180528.1NP_001180529.1NP_001180546.1.
Specificity
Human SCP2. This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2; NP_001007099.1; NP_001180529.1; NP_001180528.1; NP_001180546.1 respectively),
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 28kD band in Human Adipose tissue lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0kD according to NP_001007099.1 and of 6.7kD according to NP_002970.2. The 28kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee