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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SCNN1B / ENaC Beta Antibody (C‑Terminus) IHC‑plus™ LS‑B999
Note: This antibody replaces LS-C19204
ENaC Beta antibody LS-B999 is an unconjugated rabbit polyclonal antibody to ENaC Beta (SCNN1B) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B999-50
50 µg (1 mg/ml)
Unavailable

Popular SCNN1B / ENaC Beta Products

Antibody
Species: Rat
Applications: Western blot, ELISA
IF analysis of ENaC beta in rat kidney tissues using a dilution of SCNN1B / ENaC Beta antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: IHC, Western blot
Anti-SCNN1B / ENaC Beta antibody IHC staining of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9624 dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

ENaC Beta antibody LS-B999 is an unconjugated rabbit polyclonal antibody to ENaC Beta (SCNN1B) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About SCNN1B / ENaC Beta
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. P51168 NM_000336 NP_000327.2

SCNN1B Antibody, BESC1 Antibody, Beta-NaCH Antibody, Beta ENaC Antibody, Beta-ENaC Antibody, ENaC beta Antibody, ENaCb Antibody, HENaC Antibody, Liddle syndrome Antibody, RENaC Antibody, RNENACB Antibody, SCNEB Antibody, ENaCbeta Antibody


Specifications

Target
Human SCNN1B / ENaC Beta
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Goat (tested or 100% immunogen sequence identity)
Predicted
Guinea pig, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.13 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from C-Terminus of human SCNN1B (P51168, NP_000327). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Goat, Elephant, Bovine, Bat, Opossum (100%); Gorilla, Rabbit, Horse, Guinea pig, Platypus (92%); Mouse, Rat, Hamster (85%).
Blocking Peptide
LS-E12444 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B999. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human SCNN1B
Usage
Immunohistochemistry: LS-B999 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B999 was determined to be 2.5 ug/ml.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-SCNN1B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B999 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SCNN1B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B999 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SCNN1B antibody LS-B999 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SCNN1B antibody LS-B999 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SCNN1B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B999 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SCNN1B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B999 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SCNN1B antibody LS-B999 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SCNN1B antibody LS-B999 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SCNN1B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B999 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SCNN1B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B999 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SCNN1B antibody LS-B999 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SCNN1B antibody LS-B999 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SCNN1B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B999 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SCNN1B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B999 concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SCNN1B antibody LS-B999 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SCNN1B antibody LS-B999 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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