Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to SARS Coronavirus SARS Membrane Protein
Immunofluorescence, Western blot, Immunoprecipitation
SARS Coronavirus SARS Membrane Protein
SARS Coronavirus (tested or 100% immunogen sequence identity)
Western blot (1:1000)
Specificity and Use
BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 204-221) of the SARS Coronavirus Membrane protein.
SARS-Coronavirus membrane protein. Cross reactivity with homologs from other sources has not been determined
This antibody has been tested for use in immunofluorescence microscopy, immunoprecipitation, immunoelectron microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 25 kD in size corresponding to SARS-CoV membrane protein by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. IMMUNO PRECIPITATION 1:60; IMMUNO ELECTRON MICROSCOPY 1:200;
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Anti-SARS-CoV Membrane Protein Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using anti-SARS-CoV Membrane protein antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX ( was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.