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Anti-SARS Membrane Protein Antibody (C-Terminus) LS-C59286

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Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

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Most Popular SARS Membrane Protein Antibodies

Anti-SARS Membrane Protein Antibody (C-Terminus) LS-C59286
Rabbit Polyclonal to SARS Coronavirus SARS Membrane Protein
SARS Coronavirus
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunofluorescence Image

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Rabbit Polyclonal to SARS Coronavirus SARS Membrane Protein
SARS Coronavirus
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

SARS Coronavirus SARS Membrane Protein
Rabbit
SARS Coronavirus (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • Immunofluorescence (1:1000)
  • Western blot (1:1000)
  • Immunoprecipitation

Specificity and Use

BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 204-221) of the SARS Coronavirus Membrane protein.
C-Terminus
SARS-Coronavirus membrane protein. Cross reactivity with homologs from other sources has not been determined
This antibody has been tested for use in immunofluorescence microscopy, immunoprecipitation, immunoelectron microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 25 kD in size corresponding to SARS-CoV membrane protein by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. IMMUNO PRECIPITATION 1:60; IMMUNO ELECTRON MICROSCOPY 1:200;

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About SARS Membrane Protein


Immunofluorescence

Immunofluorescence
Anti-SARS-CoV Membrane Protein Antibody - Immunofluorescence Microscopy. Immunofluorescence microscopy using anti-SARS-CoV Membrane protein antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX ( was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
 

Requested From: United States
Date Requested: 12/10/2016

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