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Anti-S100B / S100 Beta Antibody (clone 9A11B9) IHC-plus™ LS-B3465

Note: This antibody replaces LS-C41506, LS-C171383

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular S100B / S100 Beta Antibodies

Anti-S100B / S100 Beta Antibody (clone 9A11B9) IHC-plus™ LS-B3465
Mouse Monoclonal [clone 9A11B9] (IgG1) to Human S100B / S100 Beta
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-S100B / S100 Beta Antibody (PE) LS-C268729
Rabbit Polyclonal (IgG) to Human S100B / S100 Beta
Human, Mouse
IHC, Western blot, Flow Cytometry, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 9A11B9] (IgG1) to Human S100B / S100 Beta
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human S100B / S100 Beta
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [9A11B9]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:200)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

S100B / S100 Beta antibody was raised against recombinant Human S100B.
Purified full-length recombinant S100B-GST expressed in E. Coli strain BL21 (DE3)
Immunohistochemistry: LS-B3465 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3465 was determined to be 1:200.

Packaging

Ascitic fluid, 0.03% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: -20°C
For research use only.

About S100B / S100 Beta

P04271 NM_006272 NP_006263.1

S100B Antibody, NEF Antibody, Protein S100-B Antibody, S100 Antibody, S100 calcium binding protein B Antibody, S100 calcium-binding protein B Antibody, S100beta Antibody, S-100 protein beta chain Antibody, S-100 protein subunit beta Antibody, S100-B Antibody

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3.

Immunohistochemistry

Immunohistochemistry
Anti-S100B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3465 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-S100B antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3465 dilution 1:200.

Western blot

Western blot
Western blot using S100B mouse monoclonal antibody against full-length S100B recombinant protein.
 

Requested From: United States
Date Requested: 12/7/2016

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