Human S100A8 / MRP8

Mouse

Human, Monkey, Mouse, Rat, Cat, Dog, Guinea pig, Pig (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

MAC 387

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified from ascites

Azide-free

Affinity purified monocyte membrane preparation

Recognizes neutrophils, monocytes, macrophages and squamous mucosal epithelia. Has been shown as an important marker for identifying macrophages in tissue sections.

• IHC - Paraffin (10 µg/ml)

Immunohistochemistry: LS-B2256 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2256 was determined to be 5 ug/ml. Positive control: Tonsil, lymph node or spleen. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

10 mM PBS, pH 7.4, No preservatives added, No stabilizing proteins added

Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05109 NCBI: NM_002964 NP_002955.2