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Anti-RUNX3 Antibody LS-C150053


Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular RUNX3 Antibodies

Anti-RUNX3 Antibody (aa186-415, APC) LS-C131972
Mouse Monoclonal (IgG2a) to Human RUNX3
Flow Cytometry
APC Conjugated
Anti-RUNX3 Antibody (aa168-197) LS-C162814
Rabbit Polyclonal to Human RUNX3
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image
Anti-RUNX3 Antibody (clone 2B3) IHC-plus™ LS-B10743
Mouse Monoclonal [clone 2B3] (IgG2b) to Human RUNX3
IHC - Paraffin, ICC, Western blot, Flow Cytometry, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human RUNX3
ICC, Western blot, ELISA


Human RUNX3
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • ICC (10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

RUNX3 antibody was raised against e. coli-derived rhCBFA3 (aa 186 - 415).
Human CBFA3.
This antibody shows less than 1 % cross-reactivity with rhCBFA1 and rhCBFA2. Applications. Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect human CBFA3. The detection limit for rhCBFA3 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Immunocytochemistry-This antibody has been used at a concentration of 10 µg/ml to detect human CBFA3 in Raji cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4°C followed by Rhodamine Red-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA-This antibody can be used at 0.5-1.0 µg/ml with the appropriate secondary reagents to detect human CBFA3. The detection limit for rhCBFA3 is approximately 0.5 ng/well.


Lyophilized from 0.2 µm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1% sucrose.
Store at -20°C.
For research use only.

About RUNX3

Q13761 NM_004350 NP_004341.1

RUNX3 Antibody, Acute myeloid leukemia gene 2 Antibody, CBF-alpha-3 Antibody, PEA2 alpha C Antibody, PEBP2 alpha C Antibody, PEBP2-alpha C Antibody, PEBP2A3 Antibody, PEBP2aC Antibody, AML2 Antibody, Oncogene AML-2 Antibody, CBFA3 Antibody, PEA2-alpha C Antibody, Transcription factor AML2 Antibody

RUNX3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.

Requested From: United States
Date Requested: 10/25/2016

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