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RPL30 / Ribosomal Protein L30 Antibody (aa35‑84) LS‑C111814
Ribosomal Protein L30 antibody LS-C111814 is an unconjugated rabbit polyclonal antibody to Ribosomal Protein L30 (RPL30) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C111814-50
50 µg
Unavailable

Popular RPL30 / Ribosomal Protein L30 Products

IHC of paraffin-embedded human prostate carcinoma tissue, using RPL30 Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-RPL30 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9822 dilution 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, ICC, Western blot
Immunohistochemical analysis of RPL30 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Mouse, Rat, Bovine, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Ribosomal Protein L30 antibody LS-C111814 is an unconjugated rabbit polyclonal antibody to Ribosomal Protein L30 (RPL30) from human, mouse, rat and other species. Validated for WB.
About RPL30 / Ribosomal Protein L30
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. P62888 NM_000989 NP_000980.1

RPL30 Antibody, 60S ribosomal protein L30 Antibody, L30 Antibody, Ribosomal protein L30 Antibody


Specifications

Target
Human RPL30 / Ribosomal Protein L30
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa35-84 of human RPL30 (P62888, NP_000980). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Trout, Catfish, Salmon, Sablefish, Stickleback, Pike, Zebrafish (100%); Sea squirt (92%); Beetle, Tunicate (85%).
Blocking Peptide
LS-E20329 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111814. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human RPL30
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RPL30 antibody LS-C111814 Western blot of Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPL30 antibody LS-C111814 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RPL30 antibody LS-C111814 Western blot of Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPL30 antibody LS-C111814 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RPL30 antibody LS-C111814 Western blot of Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPL30 antibody LS-C111814 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RPL30 antibody LS-C111814 Western blot of Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RPL30 antibody LS-C111814 Western blot of Placenta lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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